| Sources | Major biological effects include inhibition of the division of
cytoplasm, reversible inhibition of cell movement, induction
of nuclear extrusion,inhibition of such processes as
phagocytosis,platelet aggregation and clot retraction,
glucose transport,19-21 thyroid secretion22 and release of growth
hormone.23,24 Cytochalasin A is sulfhydryl-reactive, and was shown
to inhibit growth and sugar uptake in a Saccharomyces strain. Cytochalasin D (Zygosporin A)2 possesses antibiotic and
antitumor activity.
A study on the nuclear extrusion of mammalian erythroblast
A study was carried out to clarify the mechanism of nuclear extrusion of mammalian erythroid cells by observing erythroblasts of rabbit under various conditions in vitro. The animals were made anemic by phenylhydrazine injection and erythroblasts were obtained from the peripheral blood and observed morphologically after a certain time of incubation. After two hour incubation at 37 degrees C, about 50% of erythroblasts were denucleated. The nuclear extrusion was remarkably suppressed by the inhibitor for electron transport system or by uncouplers for oxidative phosphorylation. It was also arrested by the inhibitor of cell movement, like cytochalasin B. In contrast, monoiodo-acetic acid, ouabain and colchicine hardly inhibited the nuclear extrusion. The observations indicated that the nuclear extrusion of mammalian erythroblast is an energy-dependent process in connection with the function of contractile microfilamentous system susceptible to cytochalasin B. |
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